Cell Reprogramming & Engineering Platform

Printer-friendly versionSend by email

Summary

This platform focuses on the generation of patient and disease specific human induced pluripotent stem cell (iPSC) lines and direct reprogrammed cells allowing CCRM to provide a portfolio of characterized cell lines that will attract global research collaborators, industry sponsors and generate early revenue. This platform will also optimize standard operating procedures (SOPs) for existing technologies, develop new reprogramming technologies, and will serve as an international partner to guide the standardization and characterization of reprogrammed cells.

 

Cell Reprogramming & Engineering Capabilities

 

Establishment of cell lines

  • Tissue biopsies or blood samples are obtained under consent for the establishment of cell cultures (i.e. B-cells, T-cells, fibroblasts, endothelial, smooth muscle, epithelial cells etc.)
  • Induced Pluripotent Stem Cells (iPSCs) derived using CCRM or customer specified strategies and technologies

 

Cell banking of public, private, or specialty collections

  • Cryopreservation of primary or transformed cell lines
  • Cryopreservation of peripheral blood cells and selected tissue biopsy material
  • Maintenance of seed and distribution stocks
  • Expansion of cell lines for molecular and cytogenetic analysis
  • Database of all cell lines and associated clinical notes
 

Mycoplasma testing

  • Detection by PCR or microbiological assays
  • Identification of mycoplasma species by immunological or PCR methods
 

Remote Fail-Safe Storage

  • Cell lines
  • DNA
  • RNA
  • Protein
  • Blood products and other biomaterials
 

Reagents and Protocols

  • All reagents and protocols required for iPSC derivation and maintenance available upon request
 

Training

  • Annual course open to researchers and industry focusing on derivation, manipulation and characterization of iPSCs

 

Lead Scientists

James Ellis and Bill Stanford

 

Platform Director

Nick Timmins