Automation
- Small-scale liquid handling robot
- Enables high-throughput analytics
- Automated sample preparation
- Large-scale liquid handling robot
- Enables custom media development
- Automated cell culture maintenance
- Integrated 125-plate capacity incubator
- Enables media and buffer formulation optimization
- 96-channel head to accommodate 96-well plate
- Software enabled remote monitoring
- Integrated multi-mode cell imaging for autonomous plate quantification
Upstream Process
- A range of process-controlled bioreactor scales from 15 mL to 50 L
- Reusable glass vessels and single-use technology
- Stirred tank and rocking bed bioreactors, in various configurations, with up to 200 L capacity
- Small-scale suite of 24 bioreactors at 15 mL and 250 mL to enable rapid design of experiment
Analytics Suite
- Flow cytometers
- ddPCR
- Automated clone picker
- Confocal microscope
- Automated electrophoresis system
- High content cell analyzer
- Plate readers
- Particle Multisizer
- HPLC
Closed cell processing
- Cell harvest and washing
- Buffer exchange
- Isolation
- Viral transduction
- Expansion
- De-beading
- Concentration and reformulation
- Fill and finish
Cryopreservation equipment
- Controlled rate liquid nitrogen-free freezers
- Traditional controlled rate freezers
- -80°C freezers and LN2 vapour phase storage
Cell culture capacity
- 22 biosafety cabinets in the process development lab for a total of 15 sq ft
- 16 centrifuges including one ultracentrifuge
- 31 incubators with an average volume of 165 L
- 7 NC200 cell counters
Downstream Processing
- Processing of culture volumes ranging from 50 ml – 50 L
- Bioreactor-adapted DNA digestion protocol
- Low shear diaphragm pumps to maintain infectivity of virus
- Multiple tangential flow filtration systems, including the ability to multiplex at small scale for DoE studies
- Experience with multimodal and ion-exchange chromatography resins
- Preparative chromatography system for development of scalable processes
